Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-α Ratio in Tears of Patients with Perennial Allergic Conjunctivitis: A Preliminary Study

Autor: Enrique O Graue-Hernandez, Maria C. Jiménez-Martínez, Henry Velázquez-Soto, Jeanet Serafín-López, Israel Casanova-Méndez, Julio Ayala-Balboa, Alberto Salazar, Michele Pacheco-Quito
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
CD38
lcsh:Chemistry
0302 clinical medicine
Child
lcsh:QH301-705.5
Spectroscopy
Conjunctivitis
Allergic
B-Lymphocytes
Regulatory
biology
medicine.diagnostic_test
Interleukin
General Medicine
Allergic conjunctivitis
Computer Science Applications
Interleukin-10
Interleukin 10
IL-10
Tumor necrosis factor alpha
Female
Adolescent
Catalysis
CD19
Article
Flow cytometry
Inorganic Chemistry
03 medical and health sciences
medicine
Humans
Physical and Theoretical Chemistry
Molecular Biology
business.industry
Tumor Necrosis Factor-alpha
Organic Chemistry
tears
medicine.disease
Lymphocyte Subsets
allergic conjunctivitis
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
TNF-α
Case-Control Studies
Immunology
030221 ophthalmology & optometry
biology.protein
Tears
Mitogens
business
Bregs
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 5
International Journal of Molecular Sciences, Vol 20, Iss 5, p 1035 (2019)
ISSN: 1422-0067
Popis: Allergic conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in perennial allergic conjunctivitis (PAC), and interleukins (IL)-1&beta
IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-&alpha
were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19+IL-10+ cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19+CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-&alpha
in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with allergic conjunctivitis, characterized by the low expression of IL-10 in circulating CD19+CD38+ Bregs subsets and an inverted tear IL-10/TNF-&alpha
ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels.
Databáze: OpenAIRE
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