Effect of the PARP inhibitor veliparib on germ cell tumor cell lines
| Autor: | Silvia, Schmidtova, Natalia, Udvorkova, Zuzana, Cierna, Samuel, Horak, Katarina, Kalavska, Michal, Chovanec, Lucia, Rojikova, Miriam, Vulevova, Lucia, Kucerova, Michal, Mego |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Oncology Letters. 24 |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2022.13512 |
| Popis: | Germ cell tumors (GCTs) usually represent efficiently curable neoplasms due to their chemosensitivity to platinum-based therapeutic regimen. However, some patients develop therapeutic resistance and succumb to their disease. Novel therapeutic approaches are therefore needed for these patients. It has previously been demonstrated that poly (ADP-ribose) polymerase (PARP) expression is upregulated in GCTs compared with normal testis tissue. Therefore, PARP expression was analyzed in GCT cell lines and xenografts and it was examined whether its inhibition by veliparib can reverse cisplatin-resistance. Its expression was analyzed in sensitive and cisplatin-resistant variants (referred to as CisR throughout the manuscript) GCT cell lines and xenografts using quantitative PCR, western blotting and immunohistochemistry. The present study investigated whether the combination of cisplatin with the PARP inhibitor veliparib increased the cytotoxic effect of cisplatin |
| Databáze: | OpenAIRE |
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