hsa-miR-96 and hsa-miR-217 Expression Down-Regulates with Increasing Dysplasia in Pancreatic Intraepithelial Neoplasias and Intraductal Papillary Mucinous Neoplasms
| Autor: | Chun-Kai Yu, Xiaoyan Chang, Ji Li, Shuangni Yu, Jie Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Pathology medicine.medical_specialty endocrine system diseases Carcinogenesis In situ hybridization Intraductal papillary mucinous neoplasms Pancreatic ductal adenocarcinoma 03 medical and health sciences 0302 clinical medicine hsa-miR-96 microRNA Carcinoma medicine Humans Stage (cooking) In Situ Hybridization Aged Aged 80 and over Hyperplasia business.industry General Medicine Abnormal expression Middle Aged medicine.disease Pancreatic Intraepithelial Neoplasias hsa-miR-217 Adenocarcinoma Mucinous Pancreatic ductal epithelium Carcinoma Papillary microRNAs Gene Expression Regulation Neoplastic 030104 developmental biology Dysplasia 030220 oncology & carcinogenesis Biomarker (medicine) Female business Research Paper Carcinoma Pancreatic Ductal |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | AIM: To compare the clinicopathological features of pancreatic intraepithelial neoplasias (PanINs) and intraductal papillary mucinous neoplasms (IPMNs), and to investigate the role of hsa-miR-96 and hsa-miR-217 in these two lesions. Methods: Formalin-fixed paraffin-embedded pancreatic specimens were selected in this study, including 58 cases of pancreatic intraepithelial neoplasias (PanINs), 45 cases of pancreatic ductal adenocarcinomas (PDAs), and 57 cases of intraductal papillary mucinous neoplasms (IPMNs). MiRNAs hsa-miR-96 and hsa-miR-217 were detected using locked nucleic acid in situ hybridization (LNA-ISH) with the NBT/BCIP staining system. The differences in miRNA expression among sample sets were analyzed with the Chi-squared test. Results: PanIN-PDAs were inclined to present with higher rate of invasion (p=0.033), lymph node metastasis (p=0.0004) and poorer differentiation (p |
| Databáze: | OpenAIRE |
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