The effect of hypocapnia (PaCO2 27 mmHg) on CaM kinase IV activity, Bax/Bcl-2 protein expression and DNA fragmentation in the cerebral cortex of newborn piglets
| Autor: | Karen I. Fritz, Alan B. Zubrow, Om P. Mishra, Qazi M. Ashraf, Maria Delivoria-Papadopoulos |
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| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Swine DNA Fragmentation Biology Phosphocreatine chemistry.chemical_compound Hypocapnia Proto-Oncogene Proteins Internal medicine Ca2+/calmodulin-dependent protein kinase medicine Animals Fragmentation (cell biology) Protein kinase A bcl-2-Associated X Protein Cerebral Cortex General Neuroscience medicine.disease Endocrinology medicine.anatomical_structure Animals Newborn Gene Expression Regulation Proto-Oncogene Proteins c-bcl-2 Biochemistry chemistry Apoptosis Cerebral cortex Calcium-Calmodulin-Dependent Protein Kinases DNA fragmentation Calcium-Calmodulin-Dependent Protein Kinase Type 4 |
| Zdroj: | Neuroscience Letters. 352:211-215 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2003.08.049 |
| Popis: | The present study tests the hypothesis that a PaCO 2 of 27 mmHg for 1 hr results in increased neuronal nuclear Ca ++ /calmodulin-dependent protein kinase IV (CaM kinase IV) activity, pro-apoptotic protein expression and DNA fragmentation in the cerebral cortex of newborn piglets. Hypocapnic (HC) and normocapnic newborn piglets were studied. Tissue levels of ATP and phosphocreatine (PCr) were lower in the HC group. CaM kinase IV activity and Bax protein density were higher in the HC group. Bcl-2 protein density was the same in both groups, resulting in an increased ratio of Bax/Bcl-2 in the HC group. Density of nuclear DNA fragments was greater in the HC group and varied inversely with ATP and PCr levels. We conclude that hypocapnia (PaCO 2 27 mmHg) results in increased expression of pro-apoptotic proteins and fragmentation of nuclear DNA in newborn piglets. |
| Databáze: | OpenAIRE |
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