Cannabinoids Determination in Brain: A Supplemental Helpful in Postmortem Evaluation
| Autor: | Manuela Licata, Enrico Silingardi, Daniele Vandelli, Patrizia Verri, Filippo Marchesi, Federica Palazzoli, Anna Laura Santunione |
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| Rok vydání: | 2020 |
| Předmět: |
Analyte
Postmortem studies brain Health Toxicology and Mutagenesis Brain tissue Toxicology Tandem mass spectrometry 01 natural sciences Analytical Chemistry cannabinoids 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Postmortem mental disorders medicine Environmental Chemistry 030216 legal & forensic medicine Dronabinol Contributory factor Chemical Health and Safety Chromatography business.industry Cannabinoids 010401 analytical chemistry Brain 0104 chemical sciences Highly sensitive Autopsy business Cannabidiol medicine.drug |
| Zdroj: | Journal of analytical toxicology. 45(9) |
| ISSN: | 1945-2403 |
| Popis: | The scientific interest in cannabis has been documented by a wide literature, but postmortem studies and interpretations of autopsy findings are lacking or limited to few cases, few matrices analyzed or a small number of analytes. The present study describes the development and full in-house validation of a sensitive and simple method based on an optimized rapid clean-up procedure combined with a robust and highly sensitive liquid chromatography coupled with tandem mass spectrometry (LC–MS-MS) technique, designed to simultaneous determination of Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD), 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC), 11-nor-Δ9-tetrahydrocannabinol-carboxylic acid (THC-COOH) and 11-nor-Δ9-tetrahydrocannabinol-carboxylic acid glucuronated (THC-COOH gluc.) in postmortem samples: central blood (CB), femoral blood (FB) and brain tissue (BR). The developed method was validated and applied to 24 postmortem cases involving cannabinoids. In this study, we presented a full optimization and validation of target analyses for each matrix. The procedure had proven to be reliable and accurate. This study adds new data, particularly about the cannabinoids concentrations in BR samples. Combined pattern (CB, FB, BR) can be used in the interpretation of postmortem cases, proving and strengthening the assessments made on blood data. BR matrix is a helpful supplement in the investigation of the role of cannabinoids as crucial or contributory factor in leading to death. |
| Databáze: | OpenAIRE |
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