Array comparative genomic hybridization analysis of uterine leiomyosarcoma
| Autor: | Yong-Wan Kim, Young Lae Cho, Su-Mi Bae, Chang-Hun Lee, Heung Jae Chun, Myeong Suk Koo, Jang Heub Kim, Duck Young Ro, Chong Kook Kim, Kyung Mee Kim, Woong Shick Ahn |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Leiomyosarcoma congenital hereditary and neonatal diseases and abnormalities Gene Dosage In situ hybridization Filaggrin Proteins Biology Gene dosage chemistry.chemical_compound FGF4 medicine Humans Gene In Situ Hybridization Fluorescence Chromosome Aberrations Genetics Uterine leiomyoma medicine.diagnostic_test Nucleic Acid Hybridization Obstetrics and Gynecology DNA Neoplasm Middle Aged Molecular biology Oncology chemistry Uterine Neoplasms Female Gene Deletion DNA Fluorescence in situ hybridization Comparative genomic hybridization |
| Zdroj: | Gynecologic Oncology. 99:545-551 |
| ISSN: | 0090-8258 |
| DOI: | 10.1016/j.ygyno.2005.07.017 |
| Popis: | Purpose. Using a genome-wide array-based comparative genomic hybridization (array-CGH), DNA copy number changes in uterine leiomyosarcoma were analyzed. Materials and methods. We analyzed 4 cases of uterine leiomyoma and 7 cases of uterine leiomyosarcoma. The paraffin-fixed tissue samples were microdissected under microscope and DNA was extracted. Array-based CGH and fluorescence in situ hybridization (FISH) were carried out with Genome database (Gene Ontology). Results. Uterine leiomyoma showed no genetic alterations, while all of 7 cases of uterine leiomyosarcoma showed specific gains and losses. The percentage of average gains and losses were 4.86% and 15.1%, respectively. The regions of high level of gain were 7q36.3, 7q33–q35, 12q13–12q15, and 12q23.3. And the regions of homozygous loss were 1p21.1, 2p22.2, 6p11.2, 9p21.1, 9p21.3, 9p22.1, 14q32.33, and 14q32.33 qter. There were no recurrent regions of gain, but recurrent regions of loss were 1p21.1–p21.2, 1p22.3–p31.1, 9p21.2–p22.2, 10q25–q25.2, 11q24.2–q25, 13q12–q12.13, 14q31.1–q31.3, 14q32.32–q32.33, 15q11–q12, 15q13–q14, 18q12.1–q12.2, 18q22.1–q22.3, 20p12.1, and 21q22.12–q22.13. In the high level of gain regions, BAC clones encoded HMGIC , SAS , MDM2 , TIM1 genes. Frequently gained BAC clone-encoded genes were TIM1 , PDGFR-β , REC Q4 , VAV2 , FGF4 , KLK2 , PNUTL1 , GDNF , FLG , EXT1 , WISP1 , HER-2 , and SOX18 . The genes encoded by frequently lost BAC clones were LEU1 , ERCC5 , THBS1 , DCC , MBD2 , SCCA1 , FVT1 , CYB5 , and ETS2/E2 . A subset of cellular processes from each gene was clustered by Gene Ontology database. Conclusion. Using array-CGH, chromosomal aberrations related to uterine leiomyosarcoma were identified. The high resolution of array-CGH combined with human genome database would give a chance to find out possible target genes present in the gained or lost clones. |
| Databáze: | OpenAIRE |
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