An oxygenated perfluorocarbon emulsion improves liver graft preservation evaluated in DCD livers of male sprague dawley rats
| Autor: | Chen Rui, Xiaolei Shi, Yitao Ding, Shanshan Zhan, Jun Wang, Cai Shizhen, Guoyi Wu, Yu Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Male
genetic structures medicine.medical_treatment Organ Preservation Solutions Cold storage chemistry.chemical_element Liver transplantation Oxygen Rats Sprague-Dawley Living Donors Animals Humans Medicine Perfluorocarbon emulsion Fluorocarbons Transplantation Chromatography business.industry Organ Preservation Liver Transplantation Rats Perfusion Liver graft Liver chemistry Emulsion Emulsions business Ex vivo Homogenization (biology) |
| Zdroj: | Transplant International. 34:2087-2097 |
| ISSN: | 1432-2277 0934-0874 |
| Popis: | The application of perfluorocarbons, which can carry large quantities of oxygen, in organ preservation was limited by their poor solubility in water. A stable form of perfluorocarbon dispersed in suitable buffers is urgently needed. Perfluorocarbon emulsion was designed and characterized with respect to size distribution, rheology, stability, and oxygen-carrying capacity. The state of DCD rat donor livers preserved by the oxygenated perfluorocarbon emulsion was studied after ex vivo reperfusion by using biochemistry, pathology, and immunohistochemistry methods. Perfluorocarbon emulsion was successfully prepared by high-pressure homogenization. Optimized perfluorocarbon emulsion showed nanoscale size distribution, good stability, and higher oxygen loading capacity than that of HTK solution or water. The state of preserved livers after cardiac death rat liver was improved significantly after static cold storage for 48 hours in this oxygenated perfluorocarbon emulsion. The ATP content and down-regulation of HIF-1a expression after preservation of the liver graft by the oxygenated perfluorocarbon emulsion suggested the advantage of adequate oxygen supply for adequate time. This perfluorocarbon emulsion reported here might be considered a promising system for oxygenated donor liver storage by attenuation of hypoxia. |
| Databáze: | OpenAIRE |
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