Unexpected mutual regulation underlies paralogue functional diversification and promotes epithelial tissue maturation in Tribolium
| Autor: | Iris M. Vargas Jentzsch, Kristen A. Panfilio, Daniela Gurska |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
animal structures
Transcription Genetic Morphogenesis Medicine (miscellaneous) Genes Insect Biology Epithelium Article General Biochemistry Genetics and Molecular Biology Conserved sequence 03 medical and health sciences 0302 clinical medicine RNA interference Phylogenetics Animals lcsh:QH301-705.5 Psychological repression Gene Conserved Sequence Phylogeny 030304 developmental biology Regulation of gene expression Tribolium 0303 health sciences Sequence Analysis RNA QH fungi Gene Expression Regulation Developmental Cell biology lcsh:Biology (General) Differentiation Embryogenesis Molecular evolution Subfunctionalization Evolutionary developmental biology General Agricultural and Biological Sciences 030217 neurology & neurosurgery |
| Zdroj: | Communications Biology, Vol 3, Iss 1, Pp 1-15 (2020) Communications Biology |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-020-01250-3 |
| Popis: | Insect Hox3/zen genes represent an evolutionary hotspot for changes in function and copy number. Single orthologues are required either for early specification or late morphogenesis of the extraembryonic tissues, which protect the embryo. The tandemly duplicated zen paralogues of the beetle Tribolium castaneum present a unique opportunity to investigate both functions in a single species. We dissect the paralogues’ expression dynamics (transcript and protein) and transcriptional targets (RNA-seq after RNAi) throughout embryogenesis. We identify an unexpected role of Tc-Zen2 in repression of Tc-zen1, generating a negative feedback loop that promotes developmental progression. Tc-Zen2 regulation is dynamic, including within co-expressed multigene loci. We also show that extraembryonic development is the major event within the transcriptional landscape of late embryogenesis and provide a global molecular characterization of the extraembryonic serosal tissue. Altogether, we propose that paralogue mutual regulation arose through multiple instances of zen subfunctionalization, leading to their complementary extant roles. Gurska et al. dissect the expression and function of zen genes (Hox3 in mammals) in the beetle Tribolium castaneum. They identify dynamic regulatory interactions of the tandemly duplicated paralogues that support extraembryonic development, under a scenario of evolutionary subfunctionalization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|