Oocysts, IgG levels and immunoblot patterns determined for Cryptosporidium parvum in bovine examined during a visit to a farm (northeastern Spain)
| Autor: | F. Freire-Santos, M.E. Ares-Mazás, A.C Causapé-Valenzuela, B Fernández-da Ponte, Caridad Sánchez-Acedo, J. Quı́lez-Cinca, C.A Vergara-Castiblanco |
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| Rok vydání: | 1999 |
| Předmět: |
Blotting
Western Group ii Antibodies Protozoan Cattle Diseases Cryptosporidiosis Antigens Protozoan Enzyme-Linked Immunosorbent Assay Serology Microbiology Feces Age Distribution Antigen Animals Fluorescent Antibody Technique Indirect Parasite Egg Count Cryptosporidium parvum General Veterinary biology Incidence Antibodies Monoclonal Cryptosporidium General Medicine Serum samples biology.organism_classification Virology Spain Immunoglobulin G Cattle Electrophoresis Polyacrylamide Gel Parasitology |
| Zdroj: | Veterinary Parasitology. 81:185-193 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/s0304-4017(98)00245-3 |
| Popis: | Single fecal and serum samples were individually collected from 101 bovines selected at random during a visit to a farm in northeastern Spain (Group I, 26 animals aged 2–36 days; Group II, 34 animals aged 1.5–4.5 months; Group III, 41 animals aged 20–24 months). Testing for the presence of Cryptosporidium parvum oocysts in feces (Monofluo Kit Cryptosporidium, Diagnostics Pasteur, France) indicated that 26% animals were infected (81% of Group I, 15% of Group II and 0% of Group III). Serological testing (ELISA for detection of specific anti-C. parvum IgG) indicated that 59% animals were seropositive (12% of Group I, 74% of Group II and 78% of Group III). Immunoblotting results indicate that cattle sera recognize C. parvum antigens of widely varying molecular weights and that the number of antigens recognized increases with age. Immunoblots revealed that some of the sera beloging to the Group I reacted with protein fractions between 15 and 20 kDa but none recognized the 21–23 kDa antigen. Only few sera in the Group II recognized the protein fraction between 15 and 20 kDa. The recognition of 21–23 kDa fraction was observed by four sera from uninfected and seropositive animals. Sera from all the seronegative Group II animals recognized few antigens and always with molecular weight greater than 50 kDa. Serum samples from both seropositive and seronegative animals beloging to the Group III recognized antigens with molecular weight ranging 15–20 kDa. Surprisingly, the protein fractions between 21 and 28 kDa reacted with approximately 30% of the sera from seropositive animals and only one of the nine sera from seronegative animals. The recognition of 42–46 kDa antigens increased with the age and only reacted with the sera from uninfected animals. |
| Databáze: | OpenAIRE |
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