Ectopic Expression of the Maize Homeobox Gene Liguleless3 Alters Cell Fates in the Leaf1
| Autor: | John E. Fowler, Randall G. Tyers, Michael Freeling, Lisa Girard, Lisa Harper, Gary J. Muehlbauer |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
DNA
Complementary DNA Plant Physiology Genetic Linkage Mutant Molecular Sequence Data Plant Science Biology Genes Plant Zea mays Gene Expression Regulation Plant Complementary DNA Gene expression Genetics Amino Acid Sequence RNA Messenger Gene Alleles DNA Primers Plant Proteins Regulation of gene expression Homeodomain Proteins Base Sequence Sequence Homology Amino Acid Genes Homeobox food and beverages Gene Expression Regulation Developmental Molecular biology Phenotype Plant Leaves RNA Plant Mutation Homeobox Ectopic expression Research Article |
| Popis: | The semidominant mutation Liguleless3-O(Lg3-O) causes a blade-to-sheath transformation at the midrib region of the maize (Zea mays L.) leaf. We isolated a full-length lg3 cDNA containing aknotted1-like family homeobox. Six Lg3-Opartial revertant alleles caused by insertion of aMutator (Mu) transposon and two deletion derivatives were isolated and used to verify that ourknotted1-like cDNA corresponds to the LG3 message. In wild-type plants the LG3 mRNA is expressed in apical regions but is not expressed in leaves. In mutant plants harboring any of three dominantlg3 alleles (Lg3-O, -Mlg, and -347), LG3 mRNA is expressed in leaf sheath tissue, indicating that the Lg3 phenotype is due to ectopic expression of the gene. The Lg3-O revertant alleles represent two classes of Lg3 phenotypes that correlate well with the level of ectopic Lg3 expression. High levels of ectopic LG3 mRNA expression results in a severe Lg3 phenotype, whereas weak ectopic Lg3 expression results in a mild Lg3 phenotype. We propose that ectopic Lg3 expression early in leaf development causes the blade-to-sheath transformation, but the level of expression determines the extent of the transformation. |
| Databáze: | OpenAIRE |
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