Production of δ-aminolevulinic acid: Characterization of murine liver 4,5-dioxo valeric acid : l-alanine aminotransferase
| Autor: | Ibrahim Z. Ades, Cindy E. Mckinnhy |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Alanine chemistry.chemical_classification Valeric acid Transamination Methylglyoxal Glyoxylate cycle Mitochondria Liver Aminolevulinic Acid Cell Fractionation Biochemistry Substrate Specificity Transaminase Mice Inbred C57BL Kinetics Mice chemistry.chemical_compound Gabaculine chemistry Animals Heme Cells Cultured Transaminases |
| Zdroj: | International Journal of Biochemistry. 23:803-810 |
| ISSN: | 0020-711X |
| DOI: | 10.1016/0020-711x(91)90063-s |
| Popis: | 1. 1. We report on the kinetic properties of murine liver 4,5-dioxovaleric acid : l -alanine ammotransferase (DOVA transaminase). 2. 2. The transamination of 4,5-dioxovaleric acid (DOVA) led to the production of δ-aminolevulinic acid. 3. 3. l -Alanine was the preferred amino group donor among the common 20 ammo acids. 4. 4. The optimum pH of the reaction was 7–8. 5. 5. A Km of 220 μM for DOVA and a Km of 970 μM for l -alanine were obtained. 6. 6. The reaction was inhibited by each of the following: glyoxylate, β-chloroalanine, methylglyoxal, δ-aminolevulinate, pyruvate, heme, and gabaculine. 7. 7. None of several xenobiotic inducers of nucrosomal mixed function oxidases tested had a significant effect on DOVA transaminase activity in studies performed with murine primary hepatocyte cultures. |
| Databáze: | OpenAIRE |
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