Anti-Inflammatory Activity of the Wild Mushroom, Echinodontium tinctorium, in RAW264.7 Macrophage Cells and Mouse Microcirculation

Autor: Peter C.K. Cheung, Mehreen Zeb, Chow H. Lee, Keith N. Egger, Sumreen Javed, Hugues B. Massicotte, Wai Ming Li, Linda E. Tackaberry, Geoffrey W. Payne, Almas Yaqoob
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
Lipopolysaccharide
medicine.drug_class
Anti-Inflammatory Agents
Pharmaceutical Science
Mannose
Polysaccharide
Nitric Oxide
01 natural sciences
Fucose
Anti-inflammatory
Article
Analytical Chemistry
Microbiology
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
Mice
lcsh:Organic chemistry
Polysaccharides
010608 biotechnology
Drug Discovery
medicine
mushroom
Animals
Physical and Theoretical Chemistry
Echinodontium tinctorium
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Mushroom
biology
British Columbia
Tumor Necrosis Factor-alpha
Macrophages
Organic Chemistry
biology.organism_classification
anti-inflammation
3. Good health
RAW 264.7 Cells
chemistry
Chemistry (miscellaneous)
Galactose
polysaccharide
Molecular Medicine
Agaricales
Histamine
Zdroj: Molecules
Volume 24
Issue 19
Molecules, Vol 24, Iss 19, p 3509 (2019)
ISSN: 1420-3049
Popis: The aim of this study was to investigate the anti-inflammatory activity of a previously un-studied wild mushroom, Echinodontium tinctorium, collected from the forests of north-central British Columbia. The lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used to study the in vitro anti-inflammatory activity. The crude alkaline extract demonstrated potent anti-inflammatory activity, and was further purified using a &ldquo
bio-activity-guided-purification&rdquo
approach. The size-exclusion and ion-exchange chromatography yielded a water-soluble anti-inflammatory polysaccharide (AIPetinc). AIPetinc has an average molecular weight of 5 kDa, and is a heteroglucan composed of mainly glucose (88.6%) with a small amount of galactose (4.0%), mannose (4.4%), fucose (0.7%), and xylose (2.3%). In in vivo settings, AIPetinc restored the histamine-induced inflammatory event in mouse gluteus maximus muscle, thus confirming its anti-inflammatory activity in an animal model. This study constitutes the first report on the bioactivity of Echinodontium tinctorium, and highlights the potential medicinal benefits of fungi from the wild forests of northern British Columbia. Furthermore, it also reiterates the need to explore natural resources for alternative treatment to modern world diseases.
Databáze: OpenAIRE
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