Post-thymic T lymphocyte maturation during ontogenesis
| Autor: | Pierre-François Piguet, C Irle, Pierre Vassalli, E Kollatte |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1981 |
| Předmět: |
T cell
T-Lymphocytes Immunology Lymphocyte Cooperation Thymus Gland Biology Lymphocyte Activation Interleukin 21 Graft vs Host Reaction Mice Lectins medicine Immunology and Allergy Cytotoxic T cell Animals Antigen-presenting cell Cells Cultured Interleukin 3 CD28 Membrane Proteins Cell Differentiation T lymphocyte Articles Natural killer T cell Molecular biology medicine.anatomical_structure Animals Newborn Antibody Formation Antigens Surface Thy-1 Antigens Lymph Nodes Spleen |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Peripheral T lymphocytes from newborn (4-6-d-old) mice, isolated from the spleen or lymph nodes, show phenotypic features of immature cortical thymocytes, such as high frequencies of proliferating cells and of peanut lectin-binding cells. These are features of peripheral T cells of recent thymic origin, as shown by in situ labeling of thymocytes and subsequent observation of the migrants to the spleen, which were mainly peanut lectin-binding cells. The function of newborn peripheral T cells was compared, on a per T cell basis, with that of thymocytes and of fully mature peripheral T cells of the adult, using preparations of newborn lymph node cells containing approximately 80% of T lymphocytes. They were strikingly (about 10-fold) less competent than adult T cells in their phytohemagglutinin responsiveness, their capacities to induce a graft vs. host reaction, to proliferate in the mixed lymphocyte reaction, and to help B lymphocytes in a humoral response in vivo and in vitro. In contrast, newborn T lymphocytes were comparable to those of adults in their capacity to generate cytotoxic T lymphocytes. No suppressive effect of newborn T lymphocytes could be demonstrated in several of these assays. These results argue for an asynchronous maturation of two T cell subsets during ontogeny and demonstrate that at least some T lymphocytes leave the thymus as immature T cells resembling cortical thymocytes and further mature at the periphery. Investigation of mice submitted to thymectomy of 5 d of age showed that these incompetent post-thymic T lymphocytes are capable of considerable expansion and maturation in the peripheral lymphoid organs in the absence of a thymic influence. |
| Databáze: | OpenAIRE |
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