Establishment and Characterization of Mesenchymal Stem Cell-Like Clonal Stem Cells from Mouse Salivary Glands
| Autor: | TacGhee Yi, Jae Yol Lim, Junghee Kim, Si Na Kim, Sun U. Song, Young-Mo Kim, Songyi Lee |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Cell type Cellular differentiation Submandibular Gland Biomedical Engineering Medicine (miscellaneous) Bioengineering Cell Separation Biology Stem cell marker Mice medicine Animals Progenitor cell Cell Proliferation Stem cell transplantation for articular cartilage repair Mice Inbred C3H Tissue Engineering Gene Expression Profiling Regeneration (biology) Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Flow Cytometry Cell biology Gene Expression Regulation Microscopy Fluorescence Hepatocytes alpha-Amylases Stem cell |
| Zdroj: | Tissue Engineering Part C: Methods. 21:447-457 |
| ISSN: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2014.0204 |
| Popis: | Successful therapy for radiation-induced salivary gland (SG) hypofunction is currently unavailable; however, tissue-specific stem cells are expected to be promising candidates for SG regeneration. Here, we present our method for the establishment of single cell-derived clonal stem cells from mouse SGs and describe their characteristics. Salivary gland-derived clonal stem cells (SGSCs) were isolated and expanded in vitro by a modified subfractionation culture method. The properties of SGSCs were examined with respect to their marker expression, gene expression, differentiation potential, and in vitro immunosuppressive activity relative to bone marrow-derived mesenchymal stem cells (BM-MSCs). SGSCs appeared to largely share the characteristics of BM-MSCs based on their marker expression, whereas they differentially expressed some genes, including AQP5, E-Cadherin, Laminin, ZO-1, and COL4. SGSCs showed the ability to differentiate into fat, bone, and cartilage cell types, as well as into α-amylase-producing and hepatocyte-like cells after appropriate induction. The in vitro immunosuppressive activity of SGSCs was found to be more potent than that of BM-MSCs. These results showed that SGSCs possess the properties of MSCs with some differential gene expression and they are salivary-specific stem cells with both epithelial and mesenchymal properties. The biological functions of SGSCs and their relevance to SG epithelial progenitor cells require further investigation. |
| Databáze: | OpenAIRE |
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