The expression, localization and function of α7 nicotinic acetylcholine receptor in rat corpus cavernosum
| Autor: | Nastaran Rahimi, Ahmad Reza Dehpour, Shahram Ejtemaei Mehr, Ali Reza Abdollahi, Tahmineh Mokhtari, Fatemeh Yarmohammadi, Hedyeh Faghir-Ghanesefat |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Atropine Guanethidine Male medicine.medical_specialty Nicotine alpha7 Nicotinic Acetylcholine Receptor Aconitine Pharmaceutical Science Adrenergic Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Phenylephrine 0302 clinical medicine Internal medicine medicine Animals Pharmacology Methyllycaconitine biology Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Antagonist Electric Stimulation Rats Nitric oxide synthase 030104 developmental biology Endocrinology NG-Nitroarginine Methyl Ester chemistry biology.protein 030217 neurology & neurosurgery medicine.drug Penis |
| Zdroj: | The Journal of pharmacy and pharmacology. 69(12) |
| ISSN: | 2042-7158 |
| Popis: | Objectives Alpha7 nicotinic acetylcholine receptor (α7-nAChR), an emerging pharmacological target for a variety of medical conditions, is expressed in the most mammalian tissues with different effects. So, this study was designed to investigate the expression, localization and effect of α7-nAChR in rat corpus cavernosum (CC). Methods & Key findings Reverse transcription polymerase chain reaction (RT-PCR) revealed that α7-nAChR was expressed in rat CC and double immunofluorescence studies demonstrated the presence of α7-nAChR in corporal neurons. The rat CC segments were mounted in organ bath chambers and contracted with phenylephrine (0.1 μm -300 μm) to investigate the relaxation effect of electrical field stimulation (EFS,10 Hz) assessed in the presence of guanethidine (adrenergic blocker, 5 μm) and atropine (muscarinic cholinergic blocker, 1 μm) to obtain non-adrenergic non-cholinergic (NANC) response. Cumulative administration of nicotine significantly potentiated the EFS-induced NANC relaxation (-log EC50 = 7.5 ± 0.057). Whereas, the potentiated NANC relaxation of nicotine was significantly inhibited with different concentrations of methyllycaconitine citrate (α7-nAChR antagonist, P < 0.05) in preincubated strips. L-NAME (non-specific nitric oxide synthase inhibitor, 1 μm) completely blocked the neurogenic relaxation induced by EFS plus nicotine. Conclusion To conclude α7-nAChR is expressed in rat CC and modulates the neurogenic relaxation response to nicotine. |
| Databáze: | OpenAIRE |
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