Effectiveness of Leaves Extract Fractions of Archidendron jiringa (Jack) I.C Nielsen Against Microbes
Autor: | Sri Wardatun, Oom Komala, Lia Puspita Sari |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
food.ingredient
Flavonoid Ethyl acetate Secondary metabolite Biochemistry Biomaterials lcsh:Chemistry chemistry.chemical_compound food medicine Environmental Chemistry Agar Food science chemistry.chemical_classification biology Chemistry fungi Glycoside food and beverages General Chemistry biology.organism_classification Antimicrobial Terpenoid lcsh:QD1-999 Archidendron medicine.drug |
Zdroj: | Molekul, Vol 14, Iss 2, Pp 110-116 (2019) |
ISSN: | 2503-0310 1907-9761 |
Popis: | Archidendron jiringa (Jack) I.C Nielsen) contains a lot of essential oils, saponins, alkaloids, terpenoids, steroids, tannins, glycosides, and flavonoids. Flavonoid is a secondary metabolite compound in a plant as antimicrobial. This study aims to determine the antimicrobial activity of fractions of ethanol 96%, n-hexan, ethyl acetate, and ethanol-water leaves extract and determine levels of favonoids . A. jiringa leaves extract were tested against Streptococus mutans , Pseudomonas aeruginosa , and Candida albicans . The research using agar disc diffusion technique for antimicrobial activity and colorimetric method to know the total of flavonoid. C hloramphenicol and ketoconazole were used as a reference standard. The result of this study shows fractions of ethanol 96%, n-hexane, ethyl acetate, and ethanol-water A. jiringa leaves extract can inhibit microbial growth of S. mutans , P. aeruginosa , and C. albicans .Total of flavonoids on the extract respectively are 1.13%, 0.494%, 2.337%, and 0.549%. determined with complementary colorimetry to each leaf extract fraction A. jiringa with aluminum chloride method. Absorption spectrum measurement using a spectrophotometer. The higher percent of flavonoids would cause greater bacteria inhibitory zone but only 0.090 for pearson correlation value. Conclusions ethyl acetate leaves extract most effective inhibit microbial growth from the other fractions. |
Databáze: | OpenAIRE |
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