Coupling of solid-phase microextraction continuous bed (monolithic) capillaries with capillary zone electrophoresis for direct analysis of drugs in biological fluids
| Autor: | Olga Kornyšova, Michał Szumski, Bogusław Buszewski, Audrius Maruška, Donatas Mickevičius, Reda Jarmalavičienė, Stanislas Krawczyk, Douglas Westerlund, Ewa Kłodzińska |
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| Rok vydání: | 2008 |
| Předmět: |
Analyte
Chromatography Chemistry Capillary action Clinical Biochemistry Analytical chemistry Electrophoresis Capillary Solid-phase microextraction Biochemistry Body Fluids Analytical Chemistry Coupling (electronics) Capillary electrophoresis Pharmaceutical Preparations Desorption Biological fluids Humans Direct analysis |
| Zdroj: | ELECTROPHORESIS. 29:1753-1760 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.200800046 |
| Popis: | Hyperlink robust biocompatible solid-phase microextraction (SPME) devices were prepared using continuous bed (monolithic) restricted-access media (RAM) as the SPME capillary insert. The RAM-based SPME approach was able to simultaneously separate proteins from a biological sample, while directly extracting the active components of caffeine, paracetamol and acetylsalicylic acid from the drug NeoCitramonum. The devices were interfaced with a CZE system and fully automated analysis for sample preconcentration, desorption, separation and quantification of analytes was evaluated. Comparative study of in-line coupled SPME-CZE using RAM and RP capillary inserts was carried out. Using an SPME (RAM) insert, the calculated caffeine, paracetamol and acetylsalicylic acid LODs in a bovine plasma sample were 0.3, 0.8 and 1.9 ng/mL, respectively. |
| Databáze: | OpenAIRE |
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