Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus
| Autor: | Maureen J. Bibb, David A. Hopwood, W P Revill, T W Yu |
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| Rok vydání: | 1994 |
| Předmět: |
Molecular Sequence Data
Anthraquinones Microbiology Genome Multienzyme Complexes Polyketide synthase Gene cluster polycyclic compounds Amino Acid Sequence Cloning Molecular Molecular Biology Gene Genetics Base Sequence Sequence Homology Amino Acid biology Streptomyces coelicolor Streptomyces griseus Sequence Analysis DNA biology.organism_classification Anti-Bacterial Agents Mutagenesis Insertional Open reading frame Acyl carrier protein Genes Bacterial Multigene Family biology.protein Naphthoquinones Research Article |
| Zdroj: | Journal of Bacteriology. 176:2627-2634 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.176.9.2627-2634.1994 |
| Popis: | A fragment of DNA was cloned from the Streptomyces griseus K-63 genome by using genes (act) for the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor as a probe. Sequencing of a 5.4-kb segment of the cloned DNA revealed a set of five gris open reading frames (ORFs), corresponding to the act PKS genes, in the following order: ORF1 for a ketosynthase, ORF2 for a chain length-determining factor, ORF3 for an acyl carrier protein, ORF5 for a ketoreductase, and ORF4 for a cyclase-dehydrase. Replacement of the gris genes with a marker gene in the S. griseus genome by using a single-stranded suicide vector propagated in Escherichia coli resulted in loss of the ability to produce griseusins A and B, showing that the five gris genes do indeed encode the type II griseusin PKS. These genes, encoding a PKS that is programmed differently from those for other aromatic PKSs so far available, will provide further valuable material for analysis of the programming mechanism by the construction and analysis of strains carrying hybrid PKS. |
| Databáze: | OpenAIRE |
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