Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression
| Autor: | Anna L. Goodsall, Jonathon D. Sedgwick, Andrew L. Ford, Eléna Foulcher |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Lymphocyte T-Lymphocytes Immunology Population Biology Immunophenotyping Antigen Antigens CD Dispase Endopeptidases medicine Immunology and Allergy Macrophage Animals IL-2 receptor education education.field_of_study Cluster of differentiation Macrophages Receptors Interleukin-2 Molecular biology Rats medicine.anatomical_structure Rats Inbred Lew CD4 Antigens Neuroglia Leukocyte Common Antigens Female |
| Zdroj: | Journal of immunological methods. 194(1) |
| ISSN: | 0022-1759 |
| Popis: | Dispase was used to digest central nervous system (CNS) tissue for isolation of the fixed tissue macrophage population (CNS microglia) and other leucocytes present. An apparent reduction in expression of some leucocyte cell surface markers was investigated further by addition of CD45b allotype-marked post-activated CD4+ T cells of known phenotype to CNS tissue preparations derived from a CD45a-expressing rat strain, before enzymatic treatment. Assessment of these T cells for expression of a range of surface molecules after completion of the isolation procedure revealed almost complete loss of expression of CD4 and CD25 and reduced expression of a number of other surface antigens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect