The Tacaribe arenavirus small zinc finger protein is required for both mRNA synthesis and genome replication
| Autor: | Daniel Kolakofsky, Sylvie Rochat, Dominique Garcin |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cell Extracts
Antibodies Viral/pharmacology Transcription Genetic Viral Proteins/metabolism Molecular Sequence Data Immunology Genome Viral Antibodies Viral Virus Replication Microbiology Genome Virus Arenaviridae/genetics/growth & development Viral Proteins Transcription (biology) Virology RNA Messenger Amino Acid Sequence RNA Viral/biosynthesis Arenaviridae Peptide sequence ddc:616 Zinc finger Messenger RNA DNA-Binding Proteins/metabolism Arenavirus Base Sequence biology Zinc Fingers RNA Messenger/biosynthesis biology.organism_classification Molecular biology DNA-Binding Proteins Viral replication Insect Science RNA Viral Research Article |
| Zdroj: | Journal of Virology, Vol. 67, No 2 (1993) pp. 807-12 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.67.2.807-812.1993 |
| Popis: | An antiserum to a peptide of the Tacaribe virus Z protein was used to determine whether this small Zn(2+)-binding protein was required for viral RNA synthesis in infected cell extracts. Specific immunodepletion of the extracts invariably reduced genome synthesis to near background levels, but strong effects on mRNA synthesis occurred only early in the infection or when mRNA synthesis was relatively weak. Our results suggest that the Z protein is required for both mRNA and genome synthesis, but in somewhat different manners. |
| Databáze: | OpenAIRE |
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