Identification of PLTP as an LXR target gene and apoE as an FXR target gene reveals overlapping targets for the two nuclear receptors
| Autor: | Peter A. Edwards, Puiying A. Mak, Heidi R. Kast-Woelbern, Andrew M. Anisfeld |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Apolipoprotein E
Receptors Cytoplasmic and Nuclear QD415-436 Biology Retinoid X receptor Biochemistry Mice Liver X receptor beta Apolipoproteins E Endocrinology Phospholipid transfer protein retinoid X receptor Animals Humans Phospholipid Transfer Proteins Promoter Regions Genetic Liver X receptor Liver X Receptors Macrophages Membrane Proteins Liver X receptor alpha Cell Biology Orphan Nuclear Receptors foam cells phospholipid transfer protein Cell biology DNA-Binding Proteins Nuclear receptor hepatocytes lipids (amino acids peptides and proteins) Farnesoid X receptor Carrier Proteins liver X receptor Transcription Factors |
| Zdroj: | Journal of Lipid Research, Vol 43, Iss 12, Pp 2037-2041 (2002) |
| ISSN: | 0022-2275 |
| Popis: | Affymetrix microarray data and Northern blot assays demonstrated that phospholipid transfer protein (PL222222222216) was induced 6-fold when either murine or human macrophages were incubated in the presence of ligands for the liver X receptor (LXR) and the retinoid X receptor. Two functional LXR response elements (LXREs) were identified and characterized in the proximal promoter of the human PLTP gene. One LXRE corresponds to a traditional direct repeat separated by 4 bp. However, the second LXRE is novel in that it corresponds to an inverted repeat separated by 1 bp, and is identical to the farnesoid X receptor response element. These studies demonstrate that PLTP is a direct target for activated LXR and farnesoid X receptor (FXR). In addition, apolipoprotein E (apoE), a known LXR target gene in macrophages, was shown to be activated in liver cells by FXR ligands. Taken together, the current data suggest that a small number of genes that currently include PLTP, apoE, and apoC-II, are induced in macrophages by activated LXR and in liver by activated FXR. |
| Databáze: | OpenAIRE |
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