Bacterial Adhesion to Protein-Coated Hydrogels
| Autor: | Alonzo D. Cook, Richard D. Sagers, William G. Pitt |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Materials science
Polymers Swine Biomedical Engineering Extended wear 02 engineering and technology Bacterial Adhesion Microbiology Biomaterials 03 medical and health sciences 0302 clinical medicine Animals Humans biology Mucins Fibrinogen Proteins Biomaterial Serum Albumin Bovine 030206 dentistry Adhesion Contact Lenses Hydrophilic 021001 nanoscience & nanotechnology biology.organism_classification N-Acetylneuraminic Acid Contact lens Pseudomonas aeruginosa Self-healing hydrogels Sialic Acids Biophysics Cattle 0210 nano-technology Gels Bacteria |
| Zdroj: | Journal of Biomaterials Applications. 8:72-89 |
| ISSN: | 1530-8022 0885-3282 |
| Popis: | Extended wear soft contact lenses have been implicated in the increased occurrence of corneal bacterial infections. This research investigated the effects of polymer chemistry, water content, and pre-sorbed proteins upon the adherence of Pseudomonas aeruginosa to model hydrogels with chemistries similar to those of extended wear soft contact lenses. The hydrogels were exposed to washed suspensions of R aeruginosa in a laminar flow cell. Albumin, fibrinogen, desialylated fibrinogen, or mucin were deposited on the hydrogels before exposure to the bacteria. Results showed that with or without protein pre-exposure, bacterial adhesion decreased as water content increased. In the presence of the sorbed protein, the number of adherent bacteria increased by about 45%, and all four proteins caused similar increases in adhesion. Bacterial adhesion was not significantly influenced by the presence of sialic acid residues in the pre-sorbed protein. |
| Databáze: | OpenAIRE |
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