High-performance liquid chromatographic microassay for l-glutamate:glyoxylate aminotransferase activity in human liver. Application in primary hyperoxaluria type 1
| Autor: | Domenico Cosseddu, Sergio Pellegrino, Franco Linari, Michele Petrarulo, Martino Marangella |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Clinical Biochemistry Glyoxylate cycle Sensitivity and Specificity Biochemistry High-performance liquid chromatography Primary hyperoxaluria Glycine Transaminase medicine Humans Alanine—glyoxylate transaminase Chromatography High Pressure Liquid Transaminases chemistry.chemical_classification Chromatography biology Biochemistry (medical) Infant General Medicine Middle Aged medicine.disease Enzyme assay Phenylhydrazines Enzyme Liver chemistry Hyperoxaluria Primary biology.protein Spectrophotometry Ultraviolet Quantitative analysis (chemistry) |
| Zdroj: | Clinica Chimica Acta. 218:193-200 |
| ISSN: | 0009-8981 |
| Popis: | A rapid and sensitive liquid chromatographic technique to determine L-glutamate: glyoxylate and aminotransferase (EC 2.6.1.4) activity in human liver is described. Homogenised tissue was incubated for 60 min in the presence of substrates and the 2-oxoglutarate generated was converted into the corresponding phenylhydrazone which was determined using reversed-phase high-performance liquid chromatography. The procedure allowed the detection of the enzyme activity expressed by 7.5 μg of liver protein, it was more sensitive and less time-consuming than the spectrophotometric procedure previously used. No significant differences were found between normal controls and patients with primary hyperoxaluria. In an 8-month-infant with primary hyperoxaluria type 1, the enzyme activity was reduced to 16% of the average control values. |
| Databáze: | OpenAIRE |
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