Detection of soluble HFE associated with soluble transferrin receptor in human serum
| Autor: | Takaaki Hosoki, Katsunori Sasaki, Seiji Tanaka, Kouhei Ohnishi, Akiei Hamano, Yutaka Kohgo, Kazuya Sato, Yoshihiro Torimoto, Hiroki Tanaka, Mikihiro Fujiya, Katsuya Ikuta |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
biology Histocompatibility Antigens Class I Membrane Proteins Transferrin receptor General Medicine Major histocompatibility complex Molecular biology Protein Structure Tertiary chemistry Transferrin Cell Line Tumor Multiprotein Complexes Hereditary hemochromatosis Receptors Transferrin MHC class I Genetics biology.protein Extracellular Humans Hemochromatosis Hemochromatosis Protein Receptor Soluble transferrin receptor |
| Zdroj: | International Journal of Molecular Medicine. 27 |
| ISSN: | 1107-3756 |
| DOI: | 10.3892/ijmm.2010.584 |
| Popis: | Hereditary hemochromatosis is an autosomal recessive disease, and 80-90% of patients exhibit Cys282Tyr or His63Asp mutations in the HFE gene. HFE, also known as major histocompatibility complex (MHC) class I-like molecule, binds to transferrin receptor 1 (TfR1) and β2-microglobulin at the cell surface, forming a complex. Some MHC class I molecules are known to be soluble, raising the possibility that HFE also has a soluble form. However, it is not known whether soluble HFE (sHFE) is present in human serum, and there has been no report on the possible binding between sHFE and soluble TfR (sTfR), which is the fragment of the extracellular domain of TfR1 released into the blood. In the present study, we purified an sTfR complex from pooled serum collected from healthy volunteers, showing that the main components of the complex are sTfR and transferrin. We also confirmed the existence of sHFE in this complex. This is the first report on the existence of sHFE in human serum. |
| Databáze: | OpenAIRE |
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