Heterologous SH3-p85β inhibits influenza A virus replication
| Autor: | Kangsheng Li, Yun Su, Yanxuan Xu, Wei-Zhong Li, Dangui Zhang, Jun Zeng, Chi Zhang, Xiaoxuan Chen, Gefei Wang, Xiang-xing Zeng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
viruses
Heterologous Down-Regulation macromolecular substances Biology Viral Nonstructural Proteins medicine.disease_cause Virus Replication environment and public health lcsh:Infectious and parasitic diseases Cell Line src Homology Domains Phosphatidylinositol 3-Kinases Dogs Virology Influenza Human Influenza A virus medicine Animals Humans lcsh:RC109-216 Phosphorylation Protein kinase B PI3K/AKT/mTOR pathway Research virus diseases Protein Subunits Infectious Diseases Viral replication Cell culture Proto-Oncogene Proteins c-akt Proto-oncogene tyrosine-protein kinase Src Protein Binding |
| Zdroj: | Virology Journal Virology Journal, Vol 7, Iss 1, p 170 (2010) |
| ISSN: | 1743-422X |
| Popis: | Phosphatidylinositol 3-kinase (PI3K)/Akt signalling pathway can support the replication of influenza A virus through binding of viral NS1 protein to the Src homology 3 (SH3) domain of p85β regulatory subunit of PI3K. Here we investigated the effect of heterologously overexpressed SH3 on the replication of different influenza A virus subtypes/strains, and on the phosphorylation of Akt in the virus-infected cells. We found that heterologous SH3 reduced replication of influenza A viruses at varying degrees in a subtype/strain-dependent manner and SH3 overexpression reduced the induction of the phosphorylation of Akt in the cells infected with PR8(H1N1) and ST364(H3N2), but not with ST1233(H1N1), Ph2246(H9N2), and Qa199(H9N2). Our results suggest that interference with the NS1-p85β interaction by heterologous SH3 can be served as a useful antiviral strategy against influenza A virus infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink