Gas chromatographic—mass spectrometric analysis of volatile amines produced by several strains of clostridium
| Autor: | J.C. Darbord, A. Rimbault, J.-L. Pons, G. Leluan |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Clostridium
Potassium hydroxide Chemical ionization Chromatography Gas Chromatography Vacuum distillation Trimethylamine General Chemistry Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Isobutylamine chemistry Ammonia Amine gas treating Amines Gas chromatography–mass spectrometry Dimethylamine |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 337:213-221 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(85)80034-7 |
| Popis: | A gas chromatographic--mass spectrometric technique is proposed for the analysis of volatile amines which were isolated from Clostridium cultures by vacuum distillation and concentrated as hydrochloride salts. Headspace sampling after alkalinization of the salts under vacuum was the most suitable for subsequent gas chromatographic analysis. With ammonia-loaded helium as carrier gas, methylamines were separated on 4.8% PEG 2OM + 0.3% potassium hydroxide on Carbopack B, and other volatile amines on 28% Pennwalt 223 + 4% potassium hydroxide on Gas-Chrom R. Bacterial volatile amines (dimethylamine, trimethylamine, isobutylamine, 3-methylbutylamine, etc.) were detected with a flame-ionization detector and identified by gas chromatography--mass spectrometry in electron-impact and chemical ionization modes. |
| Databáze: | OpenAIRE |
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