Subcellular localization of SUN2 is regulated by lamin a and Rab5
| Autor: | Ying Liang, Siu Yuen Chan, Peng Hang Chiu, Kit Yan Yip |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Rab5 GTP-Binding Proteins - genetics - metabolism
animal structures Endosome Signaling in cellular processes Immunoblotting Gene Identification and Analysis lcsh:Medicine Endosomes Biology Signal transduction Endocytosis Biochemistry Molecular Genetics Molecular cell biology Lamin Type A - genetics - metabolism Membrane Proteins - genetics - metabolism Genetics Fluorescence Resonance Energy Transfer Inner membrane Humans Immunoprecipitation Intracellular Signaling Peptides and Proteins - genetics - metabolism lcsh:Science GTPase signaling rab5 GTP-Binding Proteins Multidisciplinary integumentary system Cell Membrane lcsh:R fungi Intracellular Signaling Peptides and Proteins Colocalization Membrane Proteins Computational Biology Transfection Subcellular localization Lamin Type A Molecular biology Immunohistochemistry Cell biology embryonic structures Cytochemistry lcsh:Q SUN domain Gene Function Lamin Research Article HeLa Cells |
| Zdroj: | PLoS ONE, Vol 6, Iss 5, p e20507 (2011) PLoS ONE |
| Popis: | SUN2 is an inner nuclear membrane protein with a conserved Sad1/UNC-84 homology SUN-domain at the C-terminus. Intriguingly, SUN2 has also been reported to interact with Rab5, which localizes in early endosomes. To clarify the dual subcellular localization of SUN2, we investigated its localization in lamin A/C deficient cells rescued with lamin A or lamin C isoform, and in HeLa cells transfected with Rab5 or its mutants. We found that expression of lamin A but not lamin C partly restored the nuclear envelope localization of SUN2. SUN2 was redistributed to endosomes upon overexpression of Rab5, but remained on the nuclear envelope when the SUN domain was deleted. To explore the physiological function of SUN2 in vesicle trafficking and endocytosis, we demonstrated the colocalization of endogenous SUN2 and Rab5. Moreover, overexpression of SUN2 stimulated the uptake of transferrin while suppression of SUN2 expression attenuated the process. These findings support a role of SUN2 in endocytosis. © 2011 Liang et al. published_or_final_version |
| Databáze: | OpenAIRE |
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