Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids
| Autor: | M. Sajimol Augustine, Abdulaziz Anas, Ani V. Das, Sreekumaran Sreekanth, S. Jayalekshmi |
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| Rok vydání: | 2015 |
| Předmět: |
Luminescence
Arginine Cell Survival Inorganic chemistry chemistry.chemical_element Manganese Sulfides Conjugated system Analytical Chemistry Chitosan Mice chemistry.chemical_compound X-Ray Diffraction Spectroscopy Fourier Transform Infrared Animals Humans Amino Acids Instrumentation Spectroscopy Histidine chemistry.chemical_classification Cell Death Endocytosis Atomic and Molecular Physics and Optics Amino acid HEK293 Cells chemistry Nanocrystal Zinc Compounds Nanoparticles |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 136:327-333 |
| ISSN: | 1386-1425 |
| Popis: | Highly luminescent, manganese doped, zinc sulphide (ZnS:Mn) nanocrystals biofunctionalized with chitosan and various aminoacids such as l -citrulline, l -lysine, l -arginine, l -serine, l -histidine and glycine were synthesized by chemical capping co-precipitation method at room temperature, which is a simple and cost effective technique. The synthesized nanocrystals were structurally characterized by TEM, XRD, EDXS and FT-IR spectroscopy techniques. They possess high colloidal stability with strong orange red photoluminescence emission at 598 nm. The intensity of orange red emission has been observed to be maximum in l -citrulline capped ZnS:Mn nanocrystals in which the emission at 420 nm is effectively quenched by surface passivation due to capping. Taking into consideration the prospects of these highly luminescent, bio-compatible ZnS:Mn nanocrystals in bio-imaging applications, cytotoxicity studies were conducted to identify the capping combination which would accomplish minimum toxic effects. ZnS:Mn nanocrystals biofunctionalized with chitosan, l -citrulline, glycine, l -artginine, l -serine and l -histidine showed least toxicity up to 10 nM concentrations in mouse fibroblast L929 cells, which further confirms their cytocompatibility. Also the ZnS:Mn nanocrystals biofunctionalized with l -arginine showed maximum uptake in in vitro studies carried out in human embryonic kidney cells, HEK-293T, which shows the significant role of this particular amino acid in fetoplacental nutrition. The present study highlights the suitability of aminoacid conjugated ZnS:Mn nanocrystals, as promising candidates for biomedical applications. |
| Databáze: | OpenAIRE |
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