MS/IR, a new MS-based hyphenated method for analysis of hexuronic acid epimers in glycosaminoglycans
| Autor: | Nassiba Bagdadi, Baptiste Schindler, Sihem Melizi, Loïc Barnes, Isabelle Compagnon, Gina Renois-Predelus, Stéphane Chambert, Abdul-Rahman Allouche |
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| Přispěvatelé: | Institut Lumière Matière [Villeurbanne] (ILM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Chimie Organique et Bioorganique (COB), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Spectrophotometry
Infrared Iduronic Acid Ir laser Analytical chemistry Oligosaccharides Infrared spectroscopy 010402 general chemistry Mass spectrometry 01 natural sciences Biochemistry Glycosaminoglycan Tandem Mass Spectrometry Tetrasaccharide Monosaccharide Hyaluronic Acid Molecular Biology ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Chromatography Monosaccharides 010401 analytical chemistry Cell Biology In situ spectroscopy 3. Good health 0104 chemical sciences Solutions chemistry Epimer [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] |
| Zdroj: | Glycoconjugate Journal Glycoconjugate Journal, Springer Verlag, 2017, 34 (3), pp.421-425. ⟨10.1007/s10719-016-9741-8⟩ |
| ISSN: | 0282-0080 1573-4986 |
| Popis: | We report an original MS-based hyphenated method for the elucidation of the epimerization in GAG fragments. It consists of measuring simultaneously the MS/MS spectrum and the gas phase IR spectrum to gain direct structural information. This is possible using a customized MS instrument, modified to allow injection of a tunable IR laser inside of the instrument for in situ spectroscopy of trapped ions. The proof of principle of this approach is performed in the case of a hyaluronic acid tetrasaccharide standard. In addition, we provide the reference IR fingerprint of glucuronic and Iduronic monosaccharide standards. Remarkably, we show that the gas phase IR fingerprint of reference hexuronic acid monosaccharides proves to be transposable to oligosaccharides. Therefore, the method presented here is predictive and allows structural elucidation of unknown GAG fragments, even in the absence of referenced standards. |
| Databáze: | OpenAIRE |
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