Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
| Autor: | Djalma José Fagundes, Eduardo Rodrigues Bertoletto, Roberto Teruya, Adauto Tsutomu Ikejiri, J. C. Chaves, Frederico Somaio Neto, Paulo Roberto Bertoletto, Murched Omar Taha |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Time Factors RD1-811 Ischemia Down-Regulation Gene Expression Oxidative phosphorylation Pharmacology Biology GPX4 medicine.disease_cause Antioxidants Random Allocation Mice Gene expression medicine Animals Intestinal Mucosa Lung chemistry.chemical_classification Reverse Transcriptase Polymerase Chain Reaction Glutathione peroxidase medicine.disease Small intestine Up-Regulation Intestines Mice Inbred C57BL Disease Models Animal Oxidative Stress medicine.anatomical_structure chemistry Reperfusion Injury Immunology Surgery Transcriptome Reperfusion injury Oxidative stress |
| Zdroj: | Acta Cirurgica Brasileira, Volume: 29, Issue: 3, Pages: 186-192, Published: MAR 2014 Acta Cirúrgica Brasileira v.29 n.3 2014 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Vol 29, Iss 3, Pp 186-192 (2014) |
| Popis: | PURPOSE: To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion. METHODS: Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2^(ΔΔCt)], were considered to be biologically meaningful. RESULTS: Out of a total of 84 genes in the lung that are related to oxidative stress, 67 (79.7%) were up-regulated and 17 (20.2%) were down-regulated. Only two genes (2.3%), Lpo (lactoperoxidase) (+3.51) and Gpx4 (glutathione peroxidase) (+4.10), were expressed above the three-fold threshold, while none of the down-regulated genes were expressed outside of this threshold. CONCLUSION: The intestinal ischemia/reperfusion injury promoted a gene expression profile consisting of the positive expression of oxidative genes in a remote organ. This suggests that activate signaling pathways are implicated in both cell survival and the maintenance of genome integrity in the lung. |
| Databáze: | OpenAIRE |
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