LncRNA NRON promotes the proliferation, metastasis and EMT process in bladder cancer
| Autor: | Yaoting Gui, Jianfa Li, Shaokang Yu, Chenchen Huang, Yawen Li, Tiefu Xiong, Chengle Zhuang, Fangting Zhang, Changshui Zhuang, Jing Ye, Xinbo Huang, Zeng Zhang, Fangfang Chen |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Bladder cancer long non-coding RNA Oncogene lncRNA NRON EZH2 EMT Cancer NFAT Biology medicine.disease Long non-coding RNA Metastasis 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology Downregulation and upregulation oncogene 030220 oncology & carcinogenesis medicine Cancer research bladder cancer Research Paper |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| DOI: | 10.7150/jca.37958 |
| Popis: | Background: Bladder cancer (BC) is one of the most common malignancies world-wide with high morbidity and mortality. Long noncoding RNAs (lncRNAs) are thought to play a critical role in cancer development. LncRNA NRON, a repressor of activated T-cell nuclear factor (NFAT), has been shown to be dysregulated in many cancer types. However, the clinical significance and molecular mechanism of NRON in bladder cancer is still unknown. Methods: The expression levels of NRON in BC tissues and cell lines were tested by RT-qPCR. Survival analysis was performed to detect the correlation between NRON expression and clinical outcomes in patients with BC. The biological role of NRON in BC cells proliferation and metastasis was examined in vitro and in vivo. Results: The expression of NRON was significantly upregulated in BC specimens and cell lines compared with paired adjacent normal tissues and normal cell lines. The upregulation of NRON in bladder cancer patients was significantly associated with the depth of bladder tumor invasion and poor prognosis. Knockdown of NRON inhibited BC cells proliferation, migration, invasion and tumorigenicity. Furthermore, NRON promoted epithelial-mesenchymal transition (EMT) progression, and NRON-induced EZH2 expression contributed to this process. Conclusion: In conclusion, our results suggested that NRON acted as an oncogene and tumor biomarker for BC. |
| Databáze: | OpenAIRE |
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