DNA ligase I is associated with the 21 S complex of enzymes for DNA synthesis in HeLa cells
| Autor: | Earl F. Baril, John Goodchild, Congjun Li |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
DNA Replication
DNA Ligases DNA polymerase DNA polymerase II Molecular Sequence Data DNA ligase activity Eukaryotic DNA replication Simian virus 40 Virus Replication DNA Ligase ATP Multienzyme Complexes Genetics Humans Chromatography High Pressure Liquid chemistry.chemical_classification DNA ligase Base Sequence biology Okazaki fragments DNA replication Molecular biology chemistry DNA Viral biology.protein DNA polymerase I HeLa Cells |
| Zdroj: | Nucleic Acids Research. 22:632-638 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/22.4.632 |
| Popis: | Approximately 80% of the DNA ligase activity in HeLa cell extracts is associated with the 21 S enzyme complex that functions in simian virus 40 DNA replication in vitro (Malkas et al., Biochemistry 29, 6362-6374., 1990). The DNA ligase associated with the 21 S complex was purified extensively and its physical, enzymic and immunological properties characterized as DNA ligase I. The association of DNA ligase I with the 21 S complex of enzymes for DNA synthesis provides evidence for the physiological function of this DNA ligase in DNA replication in human cells. |
| Databáze: | OpenAIRE |
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