Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii : In silico search of regulatory sequences responsive to salt and nitrogen stress
| Autor: | Marissa Calderón-Torres, Daniela E. Castro, Miguel Murguía-Romero, Patricia E. Thomé, Antonio Peña |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:Biotechnology In silico Halotolerance Osmoregulatory mechanisms Biology Applied Microbiology and Biotechnology Salt-tolerant yeast Extremophiles 03 medical and health sciences Oxidoreductase lcsh:TP248.13-248.65 Debaryomyces hansenii lcsh:QH301-705.5 Gene Transcription factor Free tyrosine Tyrosine synthesis chemistry.chemical_classification Monooxygenase biology.organism_classification Transcriptional factors (TF) Yeast 030104 developmental biology Neutralization of free radicals Nitrogen source lcsh:Biology (General) chemistry Biochemistry Oxidative stress Regulatory sequence Irreversible damages Biotechnology |
| Zdroj: | Electronic Journal of Biotechnology, Vol 29, Iss C, Pp 1-6 (2017) |
| ISSN: | 0717-3458 |
| DOI: | 10.1016/j.ejbt.2017.06.003 |
| Popis: | Background: During saline stress, the yeast Debaryomyces hansenii has as a strategy to avoid the oxidation of proteins the synthesis of tyrosine, which reacts with nitrogen radicals to form 3-nitrotyrosine, these contributes to the high halotolerace of the yeast, because it prevents the effects of associated oxidative stress. However, it has not been determined how does D. hansenii counteract the presence of this toxic compound. In this work we evaluated the D. hansenii capacity to assimilate 3-nitrotyrosine as a unique nitrogen source, and measured its denitrase activity under NaCl stress. In order to identify putative genes related to the assimilation of 3-nitrotyrosine, we performed an in silico search in the promoter regions of D. hansenii genome. Results: We identified 15 genes whose promoter had binding sequences for transcriptional factors of sodium, nitrogen and oxidative stress with oxidoreductase and monooxygenase GO annotations. Two of these genes, DEHA2E24178g and DEHA2C00286g coding for putative denitrases and having GATA sequences, evaluated by RT-PCR, showed a high expression in sodium and nitrogen stress. Conclusions: D. hansenii can grow in presence of 3-nitrotyrosine as the only nitrogen source, and have a high especific denitrase activity to degradate 3-nitrotyrosine in 1 and 2 M NaCl stress conditions. The results suggest that given the lack of information for transcriptional factors in D. hansenii , genes identified by our in silico analysis may help to explain 3-nitrotyrosine assimilation mechanisms. Normal 0 false false false ES-MX X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman",serif; border:none; mso-ansi-language:ES-MX; mso-fareast-language:ES-MX;} |
| Databáze: | OpenAIRE |
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