Effects of caffeine and related methylxanthines on fetal mouse palates cultured in vitro
| Autor: | Saju Kawauchi, Tsuneo Kosazuma |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty Pharmacology Biology Toxicology Organ culture chemistry.chemical_compound Mice Organ Culture Techniques Theophylline In vivo Internal medicine Caffeine medicine Animals Theobromine Mice Inbred ICR Chi-Square Distribution Palate In vitro Teratology Endocrinology chemistry Xanthines Toxicity Female medicine.drug |
| Zdroj: | The Journal of toxicological sciences. 19(4) |
| ISSN: | 0388-1350 |
| Popis: | The maxillary regions of day-12.5 ICR mouse fetuses were cultured in a chemically-defined serumless medium and the effects of methylxanthine derivatives on cultured palates were studied. Explanted palates were treated for 72 hr in vitro with 0.5-2 mM caffeine (CA), 1-2 mM theophylline (TP), or 1-2 mM theobromine (TB). Although the three compounds tested did not prevent the contact of opposing palatal shelves, palatal fusion was inhibited by CA and TP at a concentration of 2 mM, and the inhibitory effect of CA was more evident than that of TP. On the other hand, TB did not exert an inhibitory effect on palatogenesis at 2 mM. Since the in vitro toxicity of the methylxanthine compounds appeared to correlate well with their in vivo teratogenic potential, the organ culture method of fetal rodent palates should be a useful tool for screening teratogenic agents, especially those causing cleft palate. |
| Databáze: | OpenAIRE |
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