Antimicrobial effect of fluoroquinolones for the eradication of nontypeable Haemophilus influenzae isolates within biofilms
| Autor: | Kiwao Watanabe, Michael A. Apicella, Chiharu Kaji, Hiroshi Watanabe |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cefotaxime
medicine.drug_class Antibiotics Erythromycin Microbial Sensitivity Tests medicine.disease_cause fluoroquinolone Gatifloxacin General Biochemistry Genetics and Molecular Biology biofilm beta-Lactamases Haemophilus influenzae Microbiology Fluoroquinolone Antibiotic Antibiotic resistance Anti-Infective Agents Ampicillin Drug Resistance Bacterial medicine otorhinolaryngologic diseases H. influenzae Biomass business.industry macrolide General Medicine biochemical phenomena metabolism and nutrition Virology Bacterial Typing Techniques ceftriaxone Biofilms business BLNAS Ampicillin Resistance medicine.drug BLNAR Fluoroquinolones |
| Zdroj: | The Tohoku journal of experimental medicine. 214(2) |
| ISSN: | 0040-8727 |
| Popis: | Biofilms can be defined as communities of microorganisms attached to a surface. Those bacterial biofilms cause serious problems, such as antibiotic resistance and medical device-related infections. Nontypeable Haemophilus influenzae (NTHi) is an important pathogen in respiratory infections, as it forms biofilms both in vitro and in vivo such as human middle ear. Recent reports indicate that otitis media, paranasal sinusitis and lower respiratory tract infections caused by Haemophilus influenzae have become more difficult to treat with oral antibiotic therapy. However, there has been no attention given to antibiotic eradication of NTHi biofilm. To investigate the antimicrobial effect of various antibiotics against NTHi biofilm formation, we conducted the following comparative study using both β-lactamase-negative ampicillin (AMP)-susceptible (BLNAS) and AMP-resistant (BLNAR) NTHi strains. In a microtiter biofilm assay, both levofloxacin and gatifloxacin, of the fluoroquinolone antibiotic group, significantly inhibited biofilm formation by BLNAS and BLNAR NTHi in a dose-dependent fashion compared to ampicillin of the penicillin antibiotic group, cefotaxime of the cephalosporin antibiotic group, and both erythromycin and clarithromycin of the macrolide antibiotic group. Furthermore, in flow cell chamber studies, confocal laser scanning microscopy counted survival bacteria in mature biofilm had been treated with gatifloxacin, ampicillin, cefotaxime and erythromycin. Only gatifloxacin completely killed the BLNAR NTHi isolates within biofilms without regard to the thickness of biofilm formation. The results of this study suggest that fluoroquinolones potentially have a role in therapy against diseases caused by both BLNAS and BLNAR NTHi isolates within biofilms. Tohoku journal of experimental medicine, 214(2), pp.121-128; 2008 |
| Databáze: | OpenAIRE |
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