Liposomal encapsulation masks genotoxicity of a chemotherapeutic agent in regulatory toxicology assessments
| Autor: | Jenolyn F. Alexander, Biana Godin, David Aguirre-Villarreal |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Salmonella typhimurium
Drug Materials science media_common.quotation_subject Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Antineoplastic Agents Bioengineering CHO Cells 02 engineering and technology 010501 environmental sciences Pharmacology medicine.disease_cause 01 natural sciences Ames test Cricetulus medicine Animals General Materials Science Doxorubicin Cytotoxicity 0105 earth and related environmental sciences media_common Liposome Microbial Viability Micronucleus Tests Mutagenicity Tests 021001 nanoscience & nanotechnology Nanotoxicology Liposomes Molecular Medicine Doxorubicin Hydrochloride 0210 nano-technology Genotoxicity medicine.drug |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 13:829-833 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2016.12.016 |
| Popis: | The burgeoning application of nanotechnology to a variety of industries including cosmetics, food, medicine and materials has led to the exploration of nanotoxicology as a trending subject of research. However the role of a nanovector, in affecting the mutagenicity of its therapeutic payload has not yet been investigated. In this study, we compare the mutagenicity of the free drug - doxorubicin hydrochloride with its nanoencapsulated form - doxorubicin loaded liposome, using conventional methods required for regulatory approval. Contrary to free doxorubicin, doxorubicin encapsulated liposome expressed a significantly lower mutant frequency in the Ames assay, and was non-genotoxic in the in vitro micronucleus assay. Further investigation of the systems' cytotoxicity and their interaction with the bacterial cell envelope, suggests that the modification of the test parameters and release of the encapsulated drug prior to the Ames test show comparable mutagenic potential of the nanotherapeutic system to a free drug. |
| Databáze: | OpenAIRE |
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