Gene expression profiling reveals transcription factor networks and subgenome bias during Brassica napus seed development
| Autor: | Vanessa Hoi, Dylan J. Ziegler, Jenna L. Kalichuk, Abolfazl Hajihassani, Nina Huynh, Isobel A. P. Parkin, Stephen J. Robinson, Mark F. Belmonte, Deirdre Khan |
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| Rok vydání: | 2021 |
| Předmět: |
Crops
Agricultural 0106 biological sciences Plant Development Plant Science Biology Genes Plant 01 natural sciences Endosperm Transcriptome 03 medical and health sciences Gene Expression Regulation Plant Gene expression Genetics Transcriptional regulation Transcription factor 030304 developmental biology 2. Zero hunger Regulation of gene expression 0303 health sciences Gene knockdown Brassica napus Gene Expression Regulation Developmental food and beverages Cell Biology Gene expression profiling Seeds Genome Plant Transcription Factors 010606 plant biology & botany |
| Zdroj: | The Plant Journal. 109:477-489 |
| ISSN: | 1365-313X 0960-7412 |
| Popis: | We profiled the global gene expression landscape across the reproductive lifecycle of Brassica napus. Comparative analysis of this nascent amphidiploid revealed the contribution of each subgenome to plant reproduction. Whole genome transcription factor networks identified BZIP11 as a transcriptional regulator of early B. napus seed development. Knockdown of BZIP11 using RNA interference resulted in a similar reduction in gene activity of predicted gene targets, and a reproductive-lethal phenotype. Global mRNA profiling revealed lower accumulation of Cn subgenome transcripts relative to the An subgenome. Subgenome-specific transcription factor networks identified distinct transcription factor families enriched in each of the An and Cn subgenome early in seed development. Analysis of laser-microdissected seed subregions further reveal subgenome expression dynamics in the embryo, endosperm, and seed coat of early stage seeds. Transcription factors predicted to be regulators encoded by the An subgenome are expressed primarily in the seed coat whereas regulators encoded by the Cn subgenome were expressed primarily in the embryo. Data suggest subgenome bias are characteristic features of the B. napus seed throughout development, and that such bias might not be universal across the embryo, endosperm, and seed coat of the developing seed. Transcriptional networks spanning both the An and Cn genomes of the whole B. napus seed can identify valuable targets for seed development research and that-omics level approaches to studying gene regulation in B. napus can benefit from both broad and high-resolution analyses. |
| Databáze: | OpenAIRE |
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