Transcription organizes euchromatin via microphase separation
| Autor: | Hilbert, L., Sato, Y., Kuznetsova, K., Bianucci, T., Kimura, Hiroshi, Jﾃｼlicher, F., Honigmann, A., Zaburdaev, V., Vastenhouw, N. L. |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Life sciences
biology 0301 basic medicine Cell biology Euchromatin RNA Stability Science Transcription Genetic Euchromatin/*genetics Biophysics General Physics and Astronomy Article General Biochemistry Genetics and Molecular Biology RNA/metabolism 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Transcription (biology) ddc:570 medicine Animals Computer Simulation Polymerase Multidisciplinary RNA General Chemistry Chromatin Cell nucleus 030104 developmental biology Histone medicine.anatomical_structure chemistry Emulsions/chemistry biology.protein Mitosis/genetics Zebrafish/embryology 030217 neurology & neurosurgery DNA Embryo Nonmammalian/metabolism |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021) Nature Communications Nature Communications, 12 (1), Article no: 1360 |
| ISSN: | 2041-1723 |
| Popis: | In eukaryotes, DNA is packed inside the cell nucleus in the form of chromatin, which consists of DNA, proteins such as histones, and RNA. Euchromatin, which is permissive for transcription, is spatially organized into transcriptionally inactive domains interspersed with pockets of transcriptional activity. While transcription and RNA have been implicated in euchromatin organization, it remains unclear how their interplay forms and maintains transcription pockets. Here we combine theory and experiment to analyze the dynamics of euchromatin organization as pluripotent zebrafish cells exit mitosis and begin transcription. We show that accumulation of RNA induces formation of transcription pockets which displace transcriptionally inactive chromatin. We propose that the accumulating RNA recruits RNA-binding proteins that together tend to separate from transcriptionally inactive euchromatin. Full phase separation is prevented because RNA remains tethered to transcribed euchromatin through RNA polymerases. Instead, smaller scale microphases emerge that do not grow further and form the typical pattern of euchromatin organization. How euchromatin organisation and transcription are related is unclear. Here, the authors observe the dynamics of euchromatin organization showing that accumulating RNA recruits RNA-binding proteins that together with transcribed euchromatin separate from non-transcribed euchromatin, forming microphases. |
| Databáze: | OpenAIRE |
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