Viable neutrophils release mitochondrial DNA to form neutrophil extracellular traps
| Autor: | Inès Schmid, Hans-Uwe Simon, Shida Yousefi, Cristina C. Mihalache, Evelyne Kozlowski |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Programmed cell death
Extracellular Traps Neutrophils Apoptosis Complement C5a Granulocyte Biology DNA Mitochondrial Neutrophil Activation 03 medical and health sciences 0302 clinical medicine Extracellular medicine Humans Molecular Biology 030304 developmental biology 0303 health sciences Granulocyte-Macrophage Colony-Stimulating Factor Cell Biology Neutrophil extracellular traps Cell biology Toll-Like Receptor 4 Histone citrullination medicine.anatomical_structure Granulocyte macrophage colony-stimulating factor 030220 oncology & carcinogenesis Reactive Oxygen Species medicine.drug |
| Zdroj: | Cell Death and Differentiation Cell death and differentiation |
| ISSN: | 1476-5403 |
| Popis: | Neutrophil extracellular traps (NETs) represent extracellular structures able to bind and kill microorganisms. It is believed that they are generated by neutrophils undergoing cell death, allowing these dying or dead cells to kill microbes. We show that, following priming with granulocyte/macrophage colony-stimulating factor (GM-CSF) and subsequent short-term toll-like receptor 4 (TLR4) or complement factor 5a (C5a) receptor stimulation, viable neutrophils are able to generate NETs. Strikingly, NETs formed by living cells contain mitochondrial, but no nuclear, DNA. Pharmacological or genetic approaches to block reactive oxygen species (ROS) production suggested that NET formation is ROS dependent. Moreover, neutrophil populations stimulated with GM-CSF and C5a showed increased survival compared with resting neutrophils, which did not generate NETs. In conclusion, mitochondrial DNA release by neutrophils and NET formation do not require neutrophil death and do also not limit the lifespan of these cells. |
| Databáze: | OpenAIRE |
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