Production and Stabilization of Specific Upregulated Long Noncoding RNA HOXD-AS2 in Glioblastomas Are Mediated by TFE3 and miR-661, Respectively
| Autor: | Yiming Qin, Yingjiao Qi, Xin Zhang, Zhiang Guan, Wei Han, Xiaozhong Peng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Organic Chemistry General Medicine Glioma Catalysis Computer Science Applications nervous system diseases Inorganic Chemistry Gene Expression Regulation Neoplastic MicroRNAs Cell Movement Cell Line Tumor glioma HOXD-AS2 TFE3 miR-661 Humans RNA Long Noncoding Physical and Theoretical Chemistry Glioblastoma Molecular Biology neoplasms Spectroscopy Cell Proliferation |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 5; Pages: 2828 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23052828 |
| Popis: | Differential expression of long noncoding RNAs (lncRNA) plays a key role in the development of gliomas. Because gliomas are the most common primary central nervous system tumor and glioblastomas have poor prognosis, it is urgent to develop new diagnostic methods. We have previously reported that lncRNA HOXD-AS2, which is specifically up-regulated in gliomas, can activate cell cycle and promote the development of gliomas. It is expected to be a new marker for molecular diagnosis of gliomas, but little is known about HOXD-AS2. Here, we demonstrate that TFE3 and miR-661 maintain the high expression level of HOXD-AS2 by regulating its production and degradation. We found that TFE3 acted as a transcription factor binding to the HOXD-AS2 promoter region and raised H3K27ac to activate HOXD-AS2. As the cytoplasmic-located lncRNA, HOXD-AS2 could be degraded by miR-661. This process was inhibited in gliomas due to the low expression of miR-661. Our study explains why HOXD-AS2 was specifically up-regulated in gliomas, helps to understand the molecular characteristics of gliomas, and provids insights for the search for specific markers in gliomas. |
| Databáze: | OpenAIRE |
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