Determination of aditoprim, a new dihydrofolate reductase inhibitor, in the plasma of cows and pigs
| Autor: | Vittorio Ascalone, Bernard M. Ludwig, Jean-Claude Jordan |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Ammonium carbonate
Quality Control Aqueous solution Chloroform Chromatography Swine Metabolite General Chemistry High-performance liquid chromatography Dihydrofolate reductase inhibitor Trimethoprim chemistry.chemical_compound chemistry Drug Stability Species Specificity Blood plasma Animals Folic Acid Antagonists Cattle Spectrophotometry Ultraviolet Chromatography High Pressure Liquid Antibacterial agent |
| Zdroj: | Journal of chromatography. 383(1) |
| Popis: | A selective and sensitive high-performance liquid chromatographic method was developed for the determination of aditoprim, a new dihydrofolate reductase inhibitor, in the plasma of cows and pigs. The compound and its internal standard were extracted with chloroform from plasma buffered at pH 9 and chromatographed on a muBondapak reversed-phase column with a mixture of acetonitrile and 0.5% ammonium carbonate aqueous solution and detected by UV absorption. Aditoprim can be quantitatively extracted from plasma. The limit for quantitative determination was ca. 0.050 micrograms/ml with a standard deviation of +/- 0.006 micrograms/ml and an accuracy of +/- 3%. The assay was linear over the concentration range 0.1-5.0 micrograms/ml (precision 0.007-0.09 micrograms/ml), and the day-to-day accuracy was better than +/- 3.5%. No interference was observed from either metabolite(s) or coadministered sulphonamides. The new procedure was compared with a microbiological assay by analysing plasma samples from pigs treated with aditoprim. The two methods gave similar results in the range 0.5-5.0 micrograms/ml. |
| Databáze: | OpenAIRE |
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