Non-enzymatic glycation of peripheral nerve proteins in human diabetics
| Autor: | Michael Donaghy, Catherine Ryle |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male medicine.medical_specialty Neurofilament Glycosylation Sural nerve Nerve Tissue Proteins Arginine chemistry.chemical_compound Myelin Dorsal root ganglion Sural Nerve Glycation Internal medicine medicine Diabetes Mellitus Humans Pentosidine Cytoskeleton Myelin Sheath Aged Aged 80 and over Chemistry Lysine Middle Aged medicine.disease Endocrinology medicine.anatomical_structure Neurology Electrophoresis Polyacrylamide Gel Female Neurology (clinical) Polyneuropathy Subcellular Fractions |
| Zdroj: | Journal of the neurological sciences. 129(1) |
| ISSN: | 0022-510X |
| Popis: | We have measured non-enzymatic glycation of proteins in the cytoskeletal and myelin fractions of nerve fascicles from human sural nerves obtained from diabetic and non-diabetic amputation specimens. Levels of the early reversible glycation adduct, measured as furosine did not differ significantly between diabetics and controls in either protein fraction. Pentosidine levels per unit protein were significantly elevated in diabetics relative to controls in both cytoskeletal (5.96 vs 4.47; p = 0.037) and myelin protein (1.35 vs 0.69; p = 0.023) fractions. Protein cross-linkage in the cytoskeletal fraction, probably due to AGEs, was also higher in diabetics than controls (504 vs 349; p = 0.057). These results show that increased AGE accumulation occurs in cytoskeletal, as well as myelin, peripheral nerve proteins in diabetics. This suggests a possible new mechanism contributing to the axonal degeneration polyneuropathy of diabetes which is based upon irreversible glycation of axonal cytoskeletal proteins causing their cross-linkage and altered function. |
| Databáze: | OpenAIRE |
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