A combination of electrochemistry and mass spectrometry to monitor the interaction of reactive species with supported lipid bilayers
| Autor: | Jan-Wilm Lackmann, Heike Kahlert, Mehdi Ravandeh, Johanna Striesow, Helena Jablonowski, V. Agmo Hernández, Kristian Wende |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antioxidant Radical medicine.medical_treatment Lipid Bilayers lcsh:Medicine 02 engineering and technology Microscopy Atomic Force Physical Chemistry Article Mass Spectrometry Plasma physics 03 medical and health sciences chemistry.chemical_compound Lipid peroxides medicine Electrochemistry Membrane lipids Lipid bilayer Author Correction lcsh:Science POPC Electrodes chemistry.chemical_classification Fysikalisk kemi Reactive oxygen species Multidisciplinary Superoxide Bilayer lcsh:R Biochemistry and Molecular Biology Electrochemical Techniques Hydrogen Peroxide 021001 nanoscience & nanotechnology Reactive Nitrogen Species 030104 developmental biology Hydroperoxyl chemistry Biophysics Phosphatidylcholines lcsh:Q Gold 0210 nano-technology Reactive Oxygen Species Oxidation-Reduction Biokemi och molekylärbiologi |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) Scientific Reports |
| Popis: | Reactive oxygen and nitrogen species (RONS), e.g. generated by cold physical plasma (CPP) or photodynamic therapy, interfere with redox signaling pathways of mammalian cells, inducing downstream consequences spanning from migratory impairment to apoptotic cell death. However, the more austere impact of RONS on cancer cells remains yet to be clarified. In the present study, a combination of electrochemistry and high-resolution mass spectrometry was developed to investigate the resilience of solid-supported lipid bilayers towards plasma-derived reactive species in dependence of their composition. A 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid bilayer was undisturbed by 200 µM H2O2 (control) but showed full permeability after CPP treatment and space-occupying oxidation products such as PoxnoPC, PAzePC, and POPC hydroperoxide were found. Electron paramagnetic resonance spectroscopy demonstrated the presence of hydroxyl radicals and superoxide anion/hydroperoxyl radicals during the treatment. In contrast, small amounts of the intramembrane antioxidant coenzyme Q10 protected the bilayer to 50% and LysoPC was the only POPC derivative found, confirming the membrane protective effect of Q10. Such, the lipid membrane composition including the presence of antioxidants determines the impact of pro-oxidant signals. Given the differences in membrane composition of cancer and healthy cells, this supports the application of cold physical plasma for cancer treatment. In addition, the developed model using the combination of electrochemistry and mass spectrometry could be a promising method to study the effect of reactive species or mixes thereof generated by chemical or physical sources. |
| Databáze: | OpenAIRE |
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