Quantitative measurement of sulforaphane, iberin and their mercapturic acid pathway metabolites in human plasma and urine using liquid chromatography–tandem electrospray ionisation mass spectrometry
| Autor: | Richard Mithen, Richard J. Middleton, P. Nicholas Shaw, Amy V. Gasper, Catharine A. Ortori, Ahmed A. Al Janobi, David A. Barrett |
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| Rok vydání: | 2006 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray Chromatography Molecular Structure Metabolite Clinical Biochemistry Reproducibility of Results Cell Biology General Medicine Urine Mass spectrometry Biochemistry Acetylcysteine Analytical Chemistry chemistry.chemical_compound chemistry Isothiocyanates Liquid chromatography–mass spectrometry Sulfoxides Humans Mercapturic acid Quantitative analysis (chemistry) Ammonium acetate Thiocyanates Chromatography Liquid |
| Zdroj: | Journal of Chromatography B. 844:223-234 |
| ISSN: | 1570-0232 |
| Popis: | A quantitative liquid chromatography positive ion electrospray tandem mass spectrometric method for the simultaneous determination of sulforaphane, iberin and their metabolites in human urine and plasma is described. The stability of the metabolites was determined in aqueous solution and in human plasma. Gradient liquid chromatographic separation was performed on a Zorbax SB-Aq 3.5 microm (100 x 2.1mm) column, using a mobile phase (flow rate 0.25 mL/min) consisting of ammonium acetate buffer at pH 4 and acetonitrile. Butyl thiocarbamoyl l-cysteine was used as internal standard. The assay was linear (r(2)>0.99) over the range of 0.03-300 microM in urine and 0.03-15 microM in plasma with intra- and inter-day assay precision ( |
| Databáze: | OpenAIRE |
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