HLA-DR-restricted T cell lines from newly diagnosed type 1 diabetic patients specific for insulinoma and normal islet beta cell proteins: lack of reactivity to glutamic acid decarboxylase
| Autor: | J.M. Tremble, E. M. Bailyes, G. C. Huang, A. M. McGREGOR, J. P. Banga, S. D. Arden, T. Kaye |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male endocrine system endocrine system diseases Swine T-Lymphocytes T cell Immunology Human leukocyte antigen Biology Autoantigens Cell Line Islets of Langerhans Mice Antigen medicine HLA-DR Animals Humans Immunology and Allergy Insulinoma B cell geography geography.geographical_feature_category Glutamate Decarboxylase Antibodies Monoclonal HLA-DR Antigens Middle Aged medicine.disease Islet Rats Molecular Weight Pancreatic Neoplasms Diabetes Mellitus Type 1 medicine.anatomical_structure Female Beta cell Research Article |
| Zdroj: | Clinical and Experimental Immunology. 102:152-158 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1111/j.1365-2249.1995.tb06649.x |
| Popis: | SUMMARY T cells reacting with pancreatic islet beta cell proteins play a pivotal role in the pathogenesis of type I diabetes in experimental animal models and man, although the islet cell autoantigens against which these T cells are directed remain to be characterized. We have previously shown the presence of disease-related antigens residing in the transplantable RIN insulinoma membranes which are recognized by T ceils from diabetic NOD mice. We now report on the establishment of CD4+, T cell lines reacting with insulinoma membranes from six newly diagnosed type 1 diabetic patients. Detailed examination of T cell lines from two patients revealed that both the lines continued to react with normal islet cell proteins and. interestingly, were also stimulated by antigens present in brain microsomes. The two T cell lines showed reactivity with different molecular weight proteins of the insulinoma membranes and both the lines were histocompatibility-linked antigen (HL A)-DR restricted. Although the insulinoma membrane preparation is known to contain glutamic acid decarboxylase (GAD), none of the six T cell lines proliferates in response to purified GAD. These T cell tines will be valuable in characterizing novel islet beta cell antigens which are likely to be implicated in type 1 diabetes. |
| Databáze: | OpenAIRE |
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