A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats
| Autor: | Salah Woubit, C. de la Fe, Armelle Peyraud, Pascale Mercier, François Thiaucourt, José B. Poveda |
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| Rok vydání: | 2003 |
| Předmět: |
Mycoplasma putrefaciens
Molecular Sequence Data Lactation Disorders medicine.disease_cause Polymerase Chain Reaction Microbiology medicine Animals Mycoplasma Infections Molecular Biology Gene Chèvre Goat Diseases Polymorphism Genetic Base Sequence biology Escherichia coli Proteins Goats Membrane Proteins Mycoplasma mycoides Outbreak Cell Biology Mycoplasma medicine.disease biology.organism_classification Isolation (microbiology) Virology Mastitis PCR L74 - Troubles divers des animaux Primer (molecular biology) Mycoplasmose Protein Kinases Sequence Alignment |
| Zdroj: | Molecular and Cellular Probes |
| ISSN: | 0890-8508 |
| Popis: | Mycoplasma putrefaciens is listed as one of the etiologic agents of the contagious agalactia syndrome by the world organisation for animal health. This species has been characterized only recently, 1974, and the number of outbreaks caused by this microorganism so far is very scarce. It induces mastitis in infected goats although other symptoms such as arthritis in adults and septicaemia in kids are also frequently described. Up to now, the identification of M. putrefaciens relied on classical isolation and identification techniques which present a number of limitations. Specific primers for PCR have been designed based on sequence comparisons of the Arc B gene among the 'Mycoplasma mycoides cluster' and related species such as Mycoplasma cottewii and Mycoplasma yeatsii. Sequence alignments confirmed the taxonomic position of M. putrefaciens, which is related to the W. mycoides cluster' but also very close to M. yeatsii. The polymorphism observed amongst the different Arc B sequences allowed the determination of a primer pair yielding a specific amplification of a 316 bp-long DNA fragment by PCR. This PCR was validated in two different laboratories with a variety of mycoplasma strains isolated from goats. This new PCR technique will be very useful for a quicker determination of M. putrefaciens strains as well as a better understanding of the prevalence of M. putrefaciens infections. (Resume d'auteur) |
| Databáze: | OpenAIRE |
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