Intracellular drug delivery inLeishmania-infected macrophages: Evaluation of saponin-loaded PLGA nanoparticles
| Autor: | W. Weyenberg, S.C. De Smedt, Roosmarijn E. Vandenbroucke, Marieke Vermeersch, Louis Maes, Annick Ludwig, Sandra Apers, Paul Cos, H. Van de Ven, A. Matheeussen |
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| Rok vydání: | 2011 |
| Předmět: |
Stereochemistry
Leishmania donovani Pharmaceutical Science Microbial Sensitivity Tests Cell Line Mice chemistry.chemical_compound Drug Delivery Systems Polylactic Acid-Polyglycolic Acid Copolymer Animals Lactic Acid Leishmania infantum Fluorescein Escin biology Pharmacology. Therapy Macrophages technology industry and agriculture Mononuclear phagocyte system biology.organism_classification In vitro PLGA chemistry Drug delivery Biophysics Nanoparticles Polyglycolic Acid Ex vivo |
| Zdroj: | Journal of drug targeting |
| ISSN: | 1029-2330 1061-186X |
| DOI: | 10.3109/1061186x.2011.595491 |
| Popis: | Drug delivery systems present an opportunity to potentiate the therapeutic effect of antileishmanial drugs. Colloidal carriers are rapidly cleared by the phagocytic cells of the reticuloendothelial system (RES), rendering them ideal vehicles for passive targeting of antileishmanials. This paper describes the development of poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) for the antileishmanial saponin β-aescin. NPs were prepared using the combined emulsification solvent evaporation/salting-out technique. Confocal microscopy was used to visualise the internalisation and intracellular trafficking of fluorescein- and nile red-labelled PLGA NPs in J774A.1 macrophages infected with GFP-transfected Leishmania donovani. The in vitro activity of aescin and aescin-loaded NPs on L. infantum was determined in the axenic model as well as in the ex vivo model. The developed PLGA NPs were monodispersed with Zave |
| Databáze: | OpenAIRE |
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