Cyclin Y inhibits plasticity-induced AMPA receptor exocytosis and LTP
| Autor: | Daniel J. Whitcomb, Jung Hwa Hong, Young Na Hur, Mikyoung Park, Eunsil Cho, Philip Regan, Hanna Kim, Dong Hyun Kim, Young Ho Suh, Kwangwook Cho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Dendritic spine
Dendritic Spines Blotting Western Long-Term Potentiation Molecular neuroscience AMPA receptor Biology Hippocampal formation Hippocampus Time-Lapse Imaging Exocytosis Article Prosencephalon Cellular neuroscience Postsynaptic potential Cyclins Animals Humans Receptors AMPA Rats Wistar Cells Cultured Neurons Multidisciplinary Microscopy Confocal Neuronal Plasticity musculoskeletal neural and ocular physiology Long-term potentiation Cell biology HEK293 Cells nervous system RNA Interference |
| Zdroj: | Scientific Reports SCIENTIFIC REPORTS(5) Cho, E, Kim, D-H, Hur, Y-N, Whitcomb, D J, Regan, P, Hong, J-H, Kim, H, Ho Suh, Y, Cho, K & Park, M 2015, ' Cyclin Y inhibits plasticity-induced AMPA receptor exocytosis and LTP ', Scientific Reports, vol. 5, 12624 . https://doi.org/10.1038/srep12624 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep12624 |
| Popis: | Cyclin Y (CCNY) is a member of the cyclin protein family, known to regulate cell division in proliferating cells. Interestingly, CCNY is expressed in neurons that do not undergo cell division. Here, we report that CCNY negatively regulates long-term potentiation (LTP) of synaptic strength through inhibition of AMPA receptor trafficking. CCNY is enriched in postsynaptic fractions from rat forebrain and is localized adjacent to postsynaptic sites in dendritic spines in rat hippocampal neurons. Using live-cell imaging of a pH-sensitive AMPA receptor, we found that during LTP-inducing stimulation, CCNY inhibits AMPA receptor exocytosis in dendritic spines. Furthermore, CCNY abolishes LTP in hippocampal slices. Taken together, our findings demonstrate that CCNY inhibits plasticity-induced AMPA receptor delivery to synapses and thereby blocks LTP, identifying a novel function for CCNY in post-mitotic cells. |
| Databáze: | OpenAIRE |
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