Micro Array-Assisted Analysis of Anti-Schistosome Glycan Antibodies Elicited by Protective Vaccination With Irradiated Cercariae
| Autor: | Steffan R L Thomas, R. Alan Wilson, Y. Y. Michelle Yang, Cornelis H. Hokke, Thomas M. Kariuki, Angela van Diepen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Glycan Antibodies Helminth Schistosomiasis baboon vaccination Immunoglobulin G 03 medical and health sciences 0302 clinical medicine Immune system Antigen Polysaccharides schistosomiasis medicine Immunology and Allergy Animals antibodies 030212 general & internal medicine Cercaria glycan-microarray irradiated cercariae anti-glycan response glycan biology Schistosoma mansoni medicine.disease Microarray Analysis vaccination Papio anubis Schistosomiasis mansoni carbohydrates (lipids) Vaccination schistosomula 030104 developmental biology Infectious Diseases Immunoglobulin M Antigens Helminth Immunology biology.protein Antibody |
| Zdroj: | Journal of Infectious Diseases, 219(10), 1671-1680 |
| Popis: | Baboons vaccinated with radiation-attenuated cercariae develop high levels of protection against schistosome infection, correlating to high antibody titres towards schistosome antigens with unknown molecular identity. Using a microarray consisting of glycans isolated from different life-stages of schistosomes, we studied the anti-glycan immunoglobulin (Ig) G and IgM responses in vaccinated and challenged baboons over a time course of 25 weeks. Anti-glycan IgM responses developed early after vaccination, but did not rise in response to later vaccinations. In contrast, anti-glycan IgG developed more slowly, but was boosted by all five subsequent vaccinations. High IgM and IgG levels against O-glycans and glycosphingolipid glycans of cercariae were observed. At the time of challenge, while most antibody levels decreased in the absence of vaccination, IgG towards a subset of glycans containing multiple-fucosylated motifs remained high until 6 weeks post-challenge during challenge parasite elimination, suggesting a possible role of this IgG in protection. |
| Databáze: | OpenAIRE |
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