Detection and localization of Epstein-Barr viral genomes in angioimmunoblastic lymphadenopathy and angioimmunoblastic lymphadenopathy-like lymphoma
| Autor: | LM Weiss, ES Jaffe, XF Liu, YY Chen, D Shibata, LJ Medeiros |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Adult
Male Herpesvirus 4 Human Genes Viral Lymphoma Sialoglycoproteins Immunology Biochemistry Polymerase Chain Reaction Antigens CD hemic and lymphatic diseases Humans Aged Leukosialin Nucleic Acid Hybridization Cell Biology Hematology Middle Aged Antigens CD20 Immunohistochemistry Antigens Differentiation B-Lymphocyte Immunoblastic Lymphadenopathy DNA Viral RNA Viral Female Lymph Nodes |
| Zdroj: | Blood. 79(7) |
| ISSN: | 0006-4971 |
| Popis: | We studied 23 cases of angioimmunoblastic lymphadenopathy (AILD) and AILD-like lymphoma for evidence of Epstein-Barr virus (EBV) using the polymerase chain reaction (PCR) and in situ hybridization studies. EBV nucleic acid sequences were found by either PCR or in situ hybridization in 96% of the cases. There was a wide range in the number of EBV-positive cells among the different cases as detected by in situ hybridization. The EBV-positive cells most often possessed nuclei of intermediate to large size. Double-labeling immunohistochemistry/in situ hybridization studies demonstrated that most of the EBV-positive cells expressed the B-lineage antigen CD20 (as detected by L26), with a minority of the EBV-positive cells stained for the T-lineage associated antigen, CD43 (as detected by Leu 22). The abnormally high amounts of EBV found in AILD and AILD-like lymphoma may be a reflection of decreased immunocompetence in these patients. The presence of EBV- positive B cells may explain the presence of B-cell clones found by others as well as the paradoxical occurrence of B-cell lymphoma in a primary T-cell lymphoproliferative disorder. |
| Databáze: | OpenAIRE |
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